Glutamine synthetase from Acetobacter diazotrophicus: properties and regulation
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چکیده
منابع مشابه
Regulation of glutamine synthetase. V. Partial purification and properties of glutamine synthetase from Bacillus licheniformis.
The glutamine synthetase of Bacillus licheniformis has been obtained at about 15% purity. Sucrose gradient centrifugation gave a molecular weight value of approximately 612,000. Both l- and d-glutamate can be utilized as substrates in the biosynthetic reaction, although the l isomer was five times more active. The requirement for adenosine triphosphate (ATP) can be partially replaced by guanosi...
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We have examined three mutants of Klebsiella aerogenes whose genetic lesions (glnB, glnD, and glnE) are in loci unlinked to the structural gene for glutamine sythetase (glnA) and in which the control of both the level and state of adenylylation of glutamine synthetase is altered. Each mutation alters a different component of the adenylylation system of glutamine synthetase [L-glutamate:ammonia ...
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The chloroplastic glutamine synthetase of spinach leaves has been purified to homogeneity using affinity chromatography. This involves a tandem ;reactive blue A-agarose' and ;reactive red-A-agarose' as the final step in the procedure. This procedure results in a yield of 18 milligrams of pure glutamine synthetase per kilogram of starting material. The purity of our enzyme has been demonstrated ...
متن کاملPurification , localization , properties and regulation of glutamine synthetase from Hyphomicrobium X
The purification, location, properties and regulation of glutamine synthetase (GS) from the facultative methylotroph Hyphomicrobium X were investigated. The enzyme was purified to homogeneity by differential centrifugation, Blue Sepharose CL-6B chromatography and Sephadex G-25 gel filtration. The specific activity of the purified enzyme was 44.7 p o l min(mg protein)l . GS was cytoplasmic in lo...
متن کاملThe respiratory system and diazotrophic activity of Acetobacter diazotrophicus PAL5.
The characteristics of the respiratory system of Acetobacter diazotrophicus PAL5 were investigated. Increasing aeration (from 0.5 to 4.0 liters of air min(-1) liter of medium(-1)) had a strong positive effect on growth and on the diazotrophic activity of cultures. Cells obtained from well-aerated and diazotrophically active cultures possessed a highly active, membrane-bound electron transport s...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2001
ISSN: 0378-1097
DOI: 10.1016/s0378-1097(01)00319-6